Database : HANSEN Search on : IMUNOELETROFORESE BIDIMENSIONAL [Subject descriptor] References found : 4 [refine] Displaying: 1 .. 4   in format [Detailed]

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Id: 13475 Author: Das, P. K; Ibrahim, A. A; Binkhuysen, F; Thi Hué, P; Qui, T. H; Rangarajan R;; Ruitenberg EJ Title: The use of subcellular components of BCG for studying host-Mycobacterium interaction in relation to leprosy ..- Source: s.l; s.n; Jul.-Aug. 1982. 19 p. ilus, tab, graf. Abstract: Mycobacterium bovis-BCG was sonified and centrifuged at 90,000g for 2h to obtain pellet (P90) and supernatant (S90), and bacilli broken by chilled X-press were fractionated into cell wall (CW), plasma membrane and cytosol. Rabbits were immunized with P90, S90 and whole sonified-broken BCG. The antigenic make-up of these antisera. These analysed by cross-immunoelectrophoresis using the antisera. These subcellular preparations were also used for detecting circulating antibodies in the sera of 45 leprosy patients by Ouchterlony´s technique and immunoelectrophoresis. Although there were many antigenic determinants common to more than one fraction, some components were found in only one fraction. Furthermore, different leprosy patients showed different patterns of antibody response to the antigens in the different fractions. In addition, these fractions were also used to assess cell-mediated immunity in mice sensitized with lyophilized whole BCG as well as with irradiated Mycobacterium. it was found that BCG, intraperitoneally injected, engedered different specifically antigen-sensitized populations of lymphocytes in the spleen and lymph node. Moreover, a certain degree of antigenic cross-reactivity was observed between BCG and M. leprae, possibly at the T-cell level. In vitro experiments suggested that the CW stimulated B cells, indicating a mitogenic ctivity leading to polyclonal activation. Finally, in vitro priming experiments established that these subcellular fractions could sensitize human peripheral lymphocytes, and upon secondary culture all primed cells were able to respond to homologous preparations but not always to heterologous preparations, thus offering a means to distinguish antigens of interest from those antigenically less complicated fractions at the T-cell level.(AU). Descriptors: MYCOBACTERIUM LEPRAE/imunol CAMUNDONGOS ENDOGÂMICOS CBA MYCOBACTERIUM BOVIS/imunol IMUNOELETROFORESE BIDIMENSIONAL HANSENIASE/imunol ANTICORPOS ANTIBACTERIAS/anal ANTIGENOS DE BACTERIAS/anal TATUS FRACOES SUBCELULARES/imunol Limits: HUMANO ANIMAL FEMININO CAMUNDONGOS COELHOS SUPPORT, NON-U.S. GOV'T Electronic Medium: http://www.ilsl.br Location: BR191.1; 01556/s

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Id: 13414 Author: Closs, O; Kronvall, G Title: Experimental murine leprosy ..- Source: s.l; s.n; 1975. 6 p. ilus, tab. Abstract: Sera from C3H and C57BL mice infected with Mycobacterium lepraemurium (MLM) and from human leprosy patients were examined for antibodies against MLM by a crossed immunoelectrophoresis (CIE) technique. Altogether antibodies against six or seven MLM antigens were found in the mouse sera. After a small inoculum of MLM, C3H mice produced more antibodies than C57BL mice. After a large inoculum both strains produced about the same amount of antibodies but showed qualitative differences in their response patterns. A serum pool from patients with lepromatous leprosy contained antibodies against six MLM antigens; five of these were identical with the antigens against which antibodies were found in infected mice. C57BL mice that had lost their delayed-type hypersensitivity to MLM during the course of a disseminated infection showed a fairly strong antibody response to three antigens and a weak response to three others. Since almost the same spectrum of antibodies, although in lower titres, could be demonstrated in C57BL mice with intact delayed-type hypersensitivity to the bacilli, these antibodies did not seem to interfere with the expression of cell-mediated immunity.(AU). Descriptors: MODELOS ANIMAIS DE DOENCAS ANTIGENOS DE BACTERIAS ANTICORPOS ANTIBACTERIAS HIPERSENSIBILIDADE TARDIA/imunol IMUNIDADE CELULAR IMUNOELETROFORESE BIDIMENSIONAL HANSENIASE/imunol CAMUNDONGOS ENDOGÂMICOS C3H/imunol CAMUNDONGOS ENDOGÂMICOS C57BL/imunol MYCOBACTERIUM LEPRAEMURIUM/imunol Limits: HUMANO ANIMAL FEMININO CAMUNDONGOS Electronic Medium: http://www.ilsl.br Location: BR191.1; 00024/s

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Id: 13413 Author: Closs, O; Mshana, R. N; Harboe, M Title: Antigenic analysis of Mycobacterium leprae ..- Source: s.l; s.n; mar. 1979. 6 p. ilus. Abstract: About twenty distinct antigenic components have been demonstrated in Mycobacterium leprae (M. leprae) by crossed immunoelectrophoresis against a rabbit antiserum produced by immunization with concentrated M. leprae antigen. This system allows a more detailed analysis of the antigenic relationship between M. leprae and other mycobacteria and a better characterization of the antigenic content of various M leprae preparations than with previously available anstisera of the antigenic content of various M. leprae preparations than with previously available antisera which reacted with far fewer components. The antibody activity in sera of patients with lepromatous leprosy was studied by incorporating the sera into the intermediate gel of the M. leprae reference system. Antibodies were found against only seven of the components. Since those compared are all known to be cross-reacting widely with antigens of other mycobacteria, it is speculated that cross-immunization may influence the antibody response in lepromatous leprosy.(AU). Descriptors: IMUNOELETROFORESE BIDIMENSIONAL SOROTIPAGEM ANTIGENOS DE BACTERIAS/anal FRAGMENTOS DE IMUNOGLOBULINAS ANTICORPOS ANTIBACTERIAS MYCOBACTERIUM LEPRAE/imunol Limits: ANIMAL COELHOS Electronic Medium: http://www.ilsl.br Location: BR191.1; 00025/s

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Id: 9847 Author: Hermans, Peter W. M; Abebe, Feseha; Kuteyi, Victor I. O; Kolk, Arend H. J; Thole, Jelle E. R; Harboe, Morten Title: Molecular and immunological characterization of the highly conserved antigen 84 from Mycobacterium tuberculosis and Mycobacterium leprae ..- Source: s.l; s.n; 1995. 7 p. ilus, tab, graf. Descriptors: HANSENIASE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM TUBERCULOSIS MYCOBACTERIUM TUBERCULOSIS SEQUENCIA DE AMINOACIDOS ANTIGENOS DE BACTÉRIAS ANTIGENOS DE BACTÉRIAS SEQUENCIA DE BASES SEQUENCIA CONSERVADA ESCHERICHIA COLI BIBLIOTECA DE GENES GENES BACTERIANOS IMUNOELETROFORESE BIDIMENSIONAL DADOS DE SEQUENCIA MOLECULAR PROTEINAS RECOMBINANTES PROTEINAS RECOMBINANTES ANALISE DE SEQUENCIA DE DNA TUBERCULOSE PULMONAR Location: BR191.1; 06822/s

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